Third-harmonic generation microscopy of undeveloped photopolymerized structures

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Polarized Third-harmonic Generation Imaging of Two-photon Photopolymerized Microstructures

Two-photon photopolymerization (TPP) has been shown to be a versatile tool for fabricating subwavelength features that are localized in three dimensions (3D) [1]. However, detection of the photopolymerized structures after polymerization is difficult as the polymerized regions exhibit poor contrast against the background when viewed under a conventional light microscope. Furthermore, the tradit...

متن کامل

Signal epidetection in third-harmonic generation microscopy of turbid media.

Third-harmonic generation (THG) imaging of thick samples or large organisms requires TH light to be epicollected through the focusing objective. In this study we first estimate the amount of backward-to-forward TH radiation created by an isolated object as a function of size and spatial frequencies in the object. Theory and model experiments indicate that no significant signal can be epidetecte...

متن کامل

Intravital third harmonic generation microscopy of collective melanoma cell invasion

Cancer cell invasion is an adaptive process based on cell-intrinsic properties to migrate individually or collectively, and their adaptation to encountered tissue structure acting as barrier or providing guidance. Whereas molecular and physical mechanisms of cancer invasion are well-studied in 3D in vitro models, their topographic relevance, classification and validation toward interstitial tis...

متن کامل

Third harmonic generation microscopy of a mouse retina

PURPOSE To demonstrate lipid-specific imaging of the retina through the use of third harmonic generation (THG), a multiphoton microscopic technique in which tissue contrast is generated from optical inhomogeneities. METHODS A custom fiber laser and multiphoton microscope was constructed and optimized for simultaneous two-photon autofluorescence (TPAF) and THG retinal imaging. Imaging was perf...

متن کامل

Imaging Cytometry of Human Leukocytes with Third Harmonic Generation Microscopy

Based on third-harmonic-generation (THG) microscopy and a k-means clustering algorithm, we developed a label-free imaging cytometry method to differentiate and determine the types of human leukocytes. According to the size and average intensity of cells in THG images, in a two-dimensional scatter plot, the neutrophils, monocytes, and lymphocytes in peripheral blood samples from healthy voluntee...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: OSA Continuum

سال: 2020

ISSN: 2578-7519

DOI: 10.1364/osac.405126